Cytiva CGT insights

AAV FastFacts Series

Need-to-know. Easy to digest. All in one place

Welcome to the AAV FastFacts series

Adeno-associated virus (AAV) has emerged as the main vector for gene therapy, with nearly half of gene therapy products in development based on AAV technology. In this series, you’ll hear from subject matter experts who will share helpful tips and tricks across the whole AAV workflow.

Cell & Gene Therapy Insights designed the FastFacts series to share information on the latest innovations in advanced therapy – in an easy-to-consume format.

Register to watch the videos and/or download the posters

Plasmid processing for mRNA, gene therapy, and other vector applications

Presented by: Henrik Ihre, PhD

You’ll learn about:

A novel three-step purification process for plasmid (p)DNA
A new high productivity multimodal resin designed specifically for pDNA purification
The comparison between legacy and novel purification process in terms of productivity
A novel fiber-based chromatography format that could offer even greater productivity gains in the future

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Leveraging lessons from the COVID-19 pandemic to accelerate the development and production of viral-based gene therapies

Presented by: Yasser Kehail

You’ll learn how to:

Optimize plasmid transfection-based production of AAV in suspension culture using single-use bioreactors
Implement scalable and robust technologies for efficient purification of biologically active AAV
Consider scalable technologies and Enterprise solutions for advancing viral vector manufacturing

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Development of a scalable AAV production process in suspension cells

Presented by: Ann-Christin Magnusson

You’ll learn about:

Optimization of AAV2 triple plasmid transfection using design of experiments (DoE)
An optimized transient transfection protocol that can be used for several different AAV serotypes to achieve high titers
Scale-up of production using Xcellerex™ XDR-10 and ReadyToProcess WAVE™ 25 bioreactor systems

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Development of a scalable adeno-associated virus purification process for gene therapy

Presented by: Åsa Hagner McWhirter

You’ll learn how to:

Maximize the recovery of the affinity purification step
Remove empty capsids without too much loss of full capsids
Avoid analytic pitfalls

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Biacore™ systems for viral vector titer analysis

Presented by: Anna Moberg

You’ll learn about:

The importance of robust, reproducible analysis of virus particles and impurities in the viral vector production process
Four assays for accurate and efficient adenovirus and AAV titer analysis using Biacore™ surface plasmon resonance (SPR) systems
Parallel assay configuration using the Biacore™ 8K system, and how it can greatly shorten analysis time

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