Biacore X100 reference portal

Back to page index

Capturing biotinylated ligands on Sensor Chip CAP

Biotin CAPture Kit with Sensor Chip CAP supports reversible capture of biotinylated ligands in Biacoresystems, offering an alternative to permanent ligand capture on Sensor Chip SA. Reagents for use with Sensor Chip CAP are provided together with the sensor chip in the Biotin CAPture Kit.

Principle

The surface of Sensor Chip CAP carries single-stranded oligonucleotide DNA covalently attached to the dextran matrix. Biotin CAPture Reagent, a conjugate of complementary oligonucleotide attached to streptavidin, is injected over the surface and is captured by hybridization of the oligonucleotide strands. The biotinylated ligand is then injected and captured by high affinity binding to the streptavidin.The surface is regenerated with an injection of 6 M guanidine hydrochloride in 0.25 M NaOH which disrupts the oligonucleotide hybridization and releases the Biotin CAPture Reagent with bound ligand. New reagent is captured for each analysis cycle.

Applicability

Biotin CAPture Kit with Sensor Chip CAP provides an alternative to Sensor Chip SA for applications where reversible ligand capture is required:

• when it is difficult to find regeneration conditions for a permanently attached ligand
• when different ligands are used within the same assay

The ligand capture capacity of Sensor Chip CAP is generally somewhat lower than that of Sensor Chip SA.

Capture using Biotin CAPture Kit may not be appropriate for work with DNA-binding proteins or DNA-processing enzymes.

Experimental protocols

Experimental details for the Biotin CAPture Kit are given in the Instructions for Use, available on the product page, under related documents.

See also

List of help files

Biotinylation for streptavidin-biotin capture