Follow our connected mAb perfusion process

We understand that evaluating new biomanufacturing technologies that promise efficiency gains is challenging. That’s why we are demonstrating our mAb process which tested performance of a new Xcellerex automatic perfusion system (APS) bioreactor, compared resin and fiber-based chromatography, and utilized sensor technology — all in a production environment that was closed and connected.

Join us on our journey. Watch this digital documentary about how we planned and executed a closed connected bioprocess from small-scale models through 50 L manufacturing. .

New - Episode 14
Considerations for effective ultrafiltration in hollow fiber devices


Project introduction
A connected mAb perfusion process case study

Gain practical insights from our actual process run. Watch our connected perfusion project and follow our step by step journey from N-1 to final product as we discuss our weekly progress. Hear valuable tips from our upstream and downstream scientists in our digital series Connecting the Dots.

Publish date: 29 September, 2020
Episode 1
Tips and Tricks for aseptic connections

Increase your confidence in aseptic connections. Join our digital series, Connecting the Dots, to hear the discussion on aseptic connections in a single-use process. Hear our recommendations for success in a connected manufacturing environment. This episode highlights an important part of running a successful connected mAb perfusion process, aseptic connections.

Publish date: 12 October, 2020
Episode 2
Reducing risk of contamination during sampling

Reduce risks in perfusion bioreactors. Watch our latest Connecting the Dots digital series to learn how to minimize contamination when sampling in a perfusion bioreactor. In this episode we discuss the common sources of contamination when sampling from a perfusion bioreactor.

Publish date: 26 October, 2020
Episode 3
Effectively managing media demands in a perfusion process

Streamline media demands in your perfusion process. Watch our digital series, Connecting the Dots, and learn how our team manages media preparation in a perfusion process project. In this episode we outline and discuss the media demands and preparation step by step.

Publish date: 9 November, 2020
Episode 4
Streamlining buffer production in a perfusion process

Witness efficiency in buffer management for perfusion systems. Watch how to streamline your process with an automated buffer management system in this episode of our digital series, Connecting the Dots. In this episode we dive deeper to understand an automated buffer management system

Publish date: 23 November, 2020
Episode 5
Simplifying connections to a perfusion bioreactor

Watch this simple tutorial in connecting a perfusion flow kit. Check in with our team to see how we did it in this episode of our Connecting the Dots digital series. If you are looking for a simplified method to aid with connecting a perfusion bioreactor to a perfusion flow kit, this episode will show you all the tips and tricks.

Publish date: 7 December, 2020
Episode 6
Critical considerations for successful perfusion operations

Streamline management of day-to-day perfusion processing for improved operations. Watch our digital series, Connecting the Dots, and gain tips and tricks from our perfusion project team.

Publish date: 11 January, 2021
Episode 7
A realistic view of daily perfusion operations

Gain valuable experience in a real, full-scale perfusion process. Watch as our team discusses their insights during our connected continuous project digital series, Connecting the Dots.

Publish date: 25 January, 2021
Episode 8
Improving perfusion productivity by maintaining cell density

Improve productivity in your perfusion bioreactor with controlled cell density. Learn how our perfusion project performed in this week’s episode of Connecting the Dots..

Publish date: 9 February, 2021
Episode 9
Harvesting efficiently in a connected perfusion process

Increase perfusion bioreactor harvest efficiency. Join our digital series, Connecting the Dots, this week to see how our harvest step went.

Publish date: 23 February, 2021
Episode 10
Simplifying connections to an ÄKTA Ready system

Learn how to streamline connections to your ÄKTA system. Join our digital series, Connecting the Dots, to get some tips and tricks from the ÄKTA experts.

Publish date: 8 March, 2021
Episode 11
Optimizing protein A chromatography in a Fibro device

Discover how to optimize your protein A capture step in a perfusion process. Join the Connecting the Dots digital series to hear some critical options to consider.

Publish date: 22 March, 2021
Episode 12
How to perform viral inactivation in a WAVE

Gain practical considerations for small scale viral inactivation in single-use. See how we did this in this episode of Connecting the Dots.

Publish date: 12 April, 2021
Episode 13
Potential impact of connecting polishing processes

Discover the potential impact of connecting your polishing steps. Hear our tips and join a discussion in his episode of connecting our Connecting the Dots digital series.

Publish date: 19 April, 2021
Episode 14
Considerations for effective ultrafiltration in hollow fiber devices

Increase your knowledge of ultra-filtration/diafiltration with a hollow fiber filter. Hear our view on sizing considerations in the latest Connecting the Dots digital series.

Publish date: 3 May, 2021
Episode 15
Valuable insights from a connected mAb perfusion process case study

Further your understanding and discover the benefits of a connected perfusion bioprocess platform and hear our lessons learned from our 50 L mAb process in the final episode of Connecting the Dots.

Publish date: 17 May, 2021

Curious about continuous biomanufacturing? Join the Cytiva project team vlog series as we document our proof of concept trial and provide insights to help you efficiently plan and execute your project .

Are you confident with your aseptic connections? Watch our vlog series connecting the dots for this week’s discussion on making aseptic connections in a single use process.

Concerned about sampling risks in a perfusion process? Watch our next connecting the dots vlog to learn how to minimize contaminations when sampling in a perfusion bioreactor.