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Protein A fiber chromatography from research to scale-up and manufacturing of monoclonal antibodies


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Protein A fiber chromatography from research to scale-up and manufacturing of monoclonal antibodies

Speaker:

By Anna Grönberg Staff Research Engineer, Cytiva

Abstract:

The new Protein A fiber chromatography format allows for quick purification of many samples because of its short cycle times. This makes the pre-packed, small-scale Protein A fiber unit a versatile tool for processes such as clone selection and up-stream cell-culture media development. The time savings in downstream processes are also significant. Screenings of different buffer conditions and lifetime studies including hundreds of cycles can be performed in less than 24 hours. On the large scale, the Protein A fiber operated in a rapid cycling manner allows full utilization of the Protein A lifetime during one batch.

Examples will be given from small-scale, where automation and a combination of capture and desalting steps will increase the throughput in up-stream process development, as well as buffer and CIP optimization in downstream process development. The compatibility of fiber units with current chromatography systems regarding system flow rates, back pressure limitations, and dead volumes will also be demonstrated. Productivity, throughput, buffer consumption, and purification performance will be compared to traditional Protein A column chromatography.

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About About Anna Grönberg


Anna Grönberg, Staff Research Engineer, joined Cytiva in 2003, and has since focused on the development of chromatography resins for the capture and polishing of mAb and related applications. As a project manager, Anna and her team developed several mAb and other protein processing applications covering Cytiva’s entire downstream product portfolio in close collaboration with customers. Throughout her career at Cytiva, Anna worked with several producers of mAbs and gained a thorough understanding on how modern technology and solutions enable more efficient biopharmaceutical production. As an expert in industrial mAb purification, Anna is currently dedicated to the development of the newly launched Fibro PrismA technology and related industrial applications.



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