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Antibody-based capture

Monoclonal or polyclonal antibodies are widely-used tools in biotechnology contexts, and specifically tailored high affinity antibodies are commercially available for many antigens.

Antibodies offer a number of advantages as capturing molecules in Biacore, in addition to their commercial availability. They are readily immobilized on the sensor surface using amine coupling without compromising their antigen-binding capacity, and most monoclonal antibodies are easily regenerated at low pH using glycine-HCl. In addition, the high selectivity of most antibodies for their specific antigens makes them powerful tools for affinity capture of ligands from impure samples. The availability of monoclonal antibodies directed against chosen epitopes on the antigen also enables ligands to be captured in controlled orientations.

Antibody-based capture relies on the specific binding between an immobilized antibody and the epitope on the antigen

See also

List of help files

Principles of ligand capture